MAR序列介导野苋菜凝集素基因在白菜中的表达

Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgenic Chinese Cabbage

以‘丰顺’白菜带柄子叶为转化受体,用带有MAR(Matrix Attachment Region)和不带MAR的两种植物表达载体进行农杆菌介导转化野苋菜凝集素基因(Amaranthus viridisL.agglutinin,AVA)获得转基因的抗蚜小白菜。分析MAR序列介导对转基因表达的影响。表明利用MAR序列介导AVA基因表达,获得转基因植株的数量比对照提高29.63%;转AVA基因白菜对桃蚜(Myzus persicae)的群体发展有一定的抑制作用,平均抑制率为55.8%;MAR序列介导AVA基因表达的转基因植株中,该基因的表达水平比对照高,并且不同转基因单株间AVA基因表达差异比对照小。

The cotyledons with petiole as acceptors, Chinese cabbage genetic transformation system mediated by Agrobacterium tumefaciens was set up and Amaranthus viridis L. agglutinin （AVA） transformed plants resistanting to Myzus persicae were acquired. The effects of MAR （matrix attachment region） on AVA gene expression in transgentic Chinese cabbage were studied with two different plant expression vectors. The number of transgenic plants was 29. 63% higher than that of the control. The bioassay of Myzus persicae revealed that transgetic plants showed more or less enhanced resistance and inhibition to population, and the average inhibition rate was 55.8%. Among the transgetic plants that AVA gene were mediated by MAR, the expression level of AVA gene was higher than that of the control, and the expression variance of AVA gene among transgetic plants was smaller as well.