百合LfMADS基因植物表达载体的构建及其功能分析

Construction of Expression Vector and Function Analysis of LfMADS Genes from Lilium

为了分析百合LfMADS1和LfMADS3基因的功能,分别将其正、反义基因插入到植物组成型双元载体pBin438中,并通过根癌农杆菌LBA4404介导转化烟草。PCR和PCR-Southern杂交结果均证明外源基因已经插入到烟草基因组中。转LfMASD1反义基因植株中1朵花的雄蕊极短、花药缺失;转LfMASD1正义基因植株中发现1个花萼变瓣的突变体。在转LfMASD3反义基因植株中发现1个植株的苞叶部分瓣化,花柄变短,另外1个植株上发现1朵花缺失1枚雄蕊;而转LfMASD3正义基因植株中没有发现变异。作者认为LfMASD1是百合花器官发育的B功能基因,LfMASD3是百合花器官发育的SEP基因,这些基因在烟草中的表现说明百合的花器官特性基因的表达模式与模式植物有所不同。

In order to analyse the function of LfMADS1 and LfMADS3 from lily, their sense and antisense plant expression vectors were constructed by inserting into the pBin438 plasmid vector, and then transformed into tobacco by Agrobacterium tumefaciens LBA4404. The results of PCR and PCR-Southern for transgenic plants showed that the foreign genes were introduced into the tobacco genome. Ectopic expression of antisense 35S： ： LfMADS1 in tobacco produced one of stamen truncated with anther; while the transformed tobaccos with sense 35S： ： LfMADS1 generated a mutant in which partial sepal was converted into petal. One of the trans- genic tobaccos with antisense 35S： ： LfMADS3 showed novel phenotype by converting partial bracts into petal, another one had a flower short of one stamen; while the transgenic tobaccos with sense 35S：： LfMADS3 were morphologically indistinguishable from wild type. The preliminary results suggested that LfMADS1 belongs to B function gene, and LfMADS3 is a SEP-like gene. In comparison to the expression of other flower organ identity genes in model plants, a different gene expression pattern could be involved in Lilium.