铁线莲‘Multi-Blue’不定芽及体细胞胚发生的初步研究

A Preliminary Study on Induction of Adventitious Buds and Embryogenesis in Clematis ‘Multi-Blue'

用‘Multi-Blue’铁线莲的幼叶、茎尖、嫩茎作为外植体进行培养,发现外植体来源以茎尖为最优,基本培养基以低盐的1/2MS为最佳。茎尖在附加0.5mg.L-1TDZ+0.01mg.L-1NAA+2%蔗糖的1/2MS固体培养基上培养1个月后芽增殖率达200.8%,且幼苗长势健壮。茎尖在附加0.8mg.L-1TDZ+0.5mg.L-1NAA+2%蔗糖的1/2MS固体培养基上培养时可形成淡黄色块状至颗粒状愈伤,经多次继代培养后可逐渐形成胚性愈伤组织,胚状体诱导率可达60%,进而获得再生植株。

In a culture experiment, shoot apexes were the best explant and 1/2 MS was the best basal medium. Adventitious buds and calli were induced from shoot apexes of Clematis ＇ Multi-Blue＇ which were cultured on solid 1/2 MS medium containing 0. 5 mg · L^-1 TDZ ＋0. 01 mg · L^-1 NAA ＋2% sucrose and 0. 8 mg· L^-1 TDZ ＋0. 5 mg · L-NAA ＋2% sucrose, respectively. The induction rate of buds was up to 200. 8% after one month and the buds were vigorous. Somatic embryos were obtained from the grey yellow lumped and granular calli through succesive transfer culture. The induction rate of somatic embryos was up to 60% , and it can be developed to normal seedlings in the same medium.