摘要
从4个番茄栽培品种（Lycopersicon esculentum Miller）中获得了Tm-2^2等位基因的同源克隆，进行序列分析，结果表明：‘沙龙’、‘渝抗2号’和‘97-4’分别与‘GCR-267’（Tm-2^2）、‘GCR-236’（Tm-2）、以及‘GCR-26’（tm-2）有100％的同源性，而樱桃番茄（Lycopersicon peruvianum．var．cerasiforme）品种‘樱红1号’与‘GCR-26’（tm-2）仅有98％的同源性；在C-端的LRR结构域中，抗病基因Tm-2。和Tm-2编码蛋白的氨基酸序列与感病基因tm-2存在624和704两个位点的氨基酸差异，即624位点的脯氨酸（P）对应亮氨酸（L）、704位点的甲硫氨酸（M）对应异亮氨酸（I）；在601—790个氨基酸的LRR结构域中，抗病基因Tm-2、Tm-2及其秘鲁种（Lycopersicon peruvianum var．dentatum）lptm-2三者均与感病基因tm-2的编码蛋白存在23个氨基酸的差异；樱桃番茄品种‘樱红1号’与含有tm-2基因的栽培种之间具有同源性关系。
Alleles of Tm-2^2 from 4 different cuhivars were cloned. Sequence analysis of them showed that： Salong, Yukang-2 and 97-4 had 100% identity to reported sequences from GCR267 （Tm-22）, GCR236 （Tm-2） and GCR26 （tm-2）, respectively; Cuhivar Yinghong 1 had 98% identity to GCR26 （tm-2） ; in LRR domain of the encoded proteins, the resistant alleles Tm-2^2 and Tm-2 have two amino acid at sites of 624 and 704 different from that of the susceptible alleles tm-2, lptm-2, in which proline （P） changed to leucine （L） and methionine （M） to isoleucine （I） ; in LRR domain of the encoded proteins between the sites of 601 and 790, 23 amino acid differences were found among Tm-2^2, Tm-2, lptm-2 and tm-2; tm-2 of Yinghong 1 （Lycopersicon esculentum var. cerasiforme） might be an orthologous gene evolved from Lycopersicon esculentum cuhivars.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第2期493-496,共4页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30170633)
教育部回国留学人员基金项目
