摘要
采用RAPD技术分析了中国茶组植物23个种质资源的遗传多样性。筛选的11个随机引物扩增出135条RAPD谱带,其中有多态带123条,平均多态性为91.11%。聚类分析表明,广南茶与大苞茶之间的遗传距离最小(0.1378),汝城白毛茶与厚轴茶之间的遗传距离最大(0.8995),应用UPGMA聚类分析法构建了DNA分子系统树图,并据此讨论了茶组植物的分类。
The genetic diversity of the tea germplasm （ Camellia Section Thea） in China was studied with RAPD method. 135 RAPD bands including 123 polymorphic bands were scored in the 23 tea germplasm tested with 11 primers selected. The average of polymorphism was 91.11%. The results of cluster analysis indicated that the minimum genetic distance （0. 1378） was between C. kwangnanica and C. grandibracteata, and the maximum genetic distance （0. 8995 ） between C. pubescens and C. crassicolumna. A dendrogram by unweighted pair group method with arithmetic mean algorithm （UPGMA） analysis was obtained. Taxonomy for Camellia Section Thea was discussed by using the dendrogram.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第2期507-508,共2页
Acta Horticulturae Sinica
基金
高等学校博士学科点专项科研基金项目(20060307024)
江苏省农业高技术研究项目(BG2003301)
关键词
茶
RAPD
遗传多样性
Tea
Camellia Section Thea
RADP
Genetic diversity
