期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

腺病毒转染CTLA4-Ig对实验性自身免疫性脑脊髓炎大鼠治疗作用的研究

Therapeutic Effect of Adenovirus-transfected CTLA4-Ig on Rats with Experimental Autoimmune Encephalomyelitis
下载PDF
导出
摘要 目的探索CTLA4-Ig转基因对实验性自身免疫性脑脊髓炎(EAE)大鼠的疗效,为临床治疗多发性硬化(MS)提供新思路。方法将表达CTLA4-Ig的重组腺病毒(AdCTLA4-Ig)注射至Wistar大鼠EAE模型侧脑室内,与地塞米松治疗组对比观察实验动物发病情况和神经电生理改变。结果AdCTLA4-Ig注射后的大鼠EAE发病情况得以改善,脑干听觉诱发电位(brainstem auditory evoked potential,BAEP)和体感诱发电位(somatosensory evoked potential,SEP)异常改变有所减轻。结论AdCTLA4-Ig对EAE大鼠有治疗作用,其疗效略优于地塞米松。 Objective To investigate the therapeutic effect of adenovirus-transfected CTLA4-Ig on rats with experimental autoimmune encephalomyelitis （EAE） and to provide possible clinical method to treat multiple sclerosis. Methods Inject adenovirus-transfected CTLA4-Ig （AdCTLA4-Ig） into the lateral cerebral ventricle of rats. Then the difference of morbidity of the disease and the neural electrophysiological changes of EAE rats between AdCTLA4-Ig injected group and metasolon treated group were observed. Results The morbidity of EAE rats, brainstem auditory evoked potential （BAEP） and somatosensory evoked potential （SEP） abnormality improved significantly after lateral cerebral ventricle injection of AdCTLA4-Ig compared with metasolon treatment. Conclusions AdCTLA4-Ig has therapeutic effect on EAE rats and its curative effect is better than metasolon.
作者 黄河清 张兴梅 陈康宁
机构地区 第三军医大学附属西南医院神经内科 宁夏解放军第五医院神经内科
出处 《中国神经免疫学和神经病学杂志》 CAS 2007年第3期137-140,共4页 Chinese Journal of Neuroimmunology and Neurology
关键词 多发性硬化 实验性自身免疫性脑脊髓炎 CTLA4-IG 脑干听觉诱发电位 体感诱发电位 multiple sclerosis experimental autoimmune encephalomyelitis CTLA4-Ig brainstem auditory evoked potential somatosensory evoked potential
分类号 R744.5 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献12

  • 1Herold KC,Vezys V,Koons A,et al.CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin[J].J Immunol,1997,158(2):984-991.
  • 2吕佩源,李中秋,陈慧芳,林嘉友.口服髓鞘素对EAE的防治作用[J].脑与神经疾病杂志,2003,11(2):75-76. 被引量：3
  • 3罗高兴,吴军,易绍萱,杨世昕,贺伟峰,周立新,张宁,陈希炜,张小蓉.重组腺病毒载体局部介导 CTLA_4Ig延长小鼠异体移植皮肤的存活[J].中华烧伤杂志,2000,16(1):37-39. 被引量：39
  • 4Kono DH,Urban JL,Horvath SJ,et al.Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL/J mice[J].J Exp Med,1988,168:213-227.
  • 5Greenfield A,Nguyen A,Kuchroo K.CD28/B7 costimulation:a review[J].Crit Rev Immunol,1998,18:389-418.
  • 6Karandikar N,Eagar N,Vanderlugt L,et al.CTLA4 downregulates epitope spreading and mediates remission in relapsing experimental autoimmune encephalomyelitis[J].J Neuroimmunol,2000,109(2):173-180.
  • 7Neville K,Dal-Canto M,Bluestone J,et al.CD28 costimulatory blockade exacerbates disease severity and accelerates epitope spreading in a virus-induced autoimmune disease[J].J Virol,2000,74(18):8349-8357.
  • 8Dieckmann D,Plottner H,Berchtold S,et al.Ex vivo isolation and characterization of CD4(+)CD25(+) T cells with regulatory properties from human blood[J].J Exp Med,2001,193:1303-1310.
  • 9Oliveira EM,Bar-Or A,Waliszewska AI,et al.CTLA4 dysregulation in the activation of myelin basic protein reactive T cells may distinguish patients with multiple sclerosis from healthy controls[J].J Autoimm,2003,20(1):71-81.
  • 10Teutsch SM,Booth DR,Bennetts BH,et al.Association of common T cell activation gene polymorphisms with multiple sclerosis in Australian patients[J].J Neuroimmunol,2004,148,(1-2):218-230.

二级参考文献9

  • 1[1]Petry KG, Boullerne AI, Pousset F, et al. Experimental allergic encephalomyelitis animal models for analyzing features of multiple sclerosis. Pathol Biol(Paris), 2000,48(1) :47～53
  • 2[2]Norton WT, Poduslo SE. Myelination in rat brain: method of myelin isolation[J]. J Neurochem, 19973,21: 749～757
  • 3[3]Kono DH, Urban JL, Horvath SJ, et al. Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL/J mice. J Exp Med, 1988,168:213～227
  • 4[4]Weiner HL. Oral tolerance: immune mechanisms and treatment of autoimmune diseases. Immunol Today, 1997,18(7) :335～343
  • 5[5]Meyer AL, Benson JM, Gienapp IE, et al. Suppression of murine chronic relapsing experimental autoimmune encephalomyelitis by the oral administration of wyelin basicprotein. J Immunol, 1996, 157(9) :4230～4238
  • 6[6]Elliott EA, Cofiell R, Wilkins JA, et al. Immune tolerance mediated by recombinant proteolipid protein prevents experimental autoimmune encephalomyelitis. J Neuroimmunol, 1997,79 : 1 ～ 11
  • 7[7]Aharoni R, Meshorer A, Sela M, et al. Oral treatment of mice with copolymer 1 (glatiramer acetate) results in the accumulation of specific Th2 cells in the central nervous system. J Neuroimmunol,2002, 126 (1～2):58～68
  • 8葛绳德，手术学、整形与烧伤外科学卷，1996年，1212页
  • 9胡嘉念，烧伤治疗学，1995年，498页

共引文献38

中国神经免疫学和神经病学杂志
2007年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部