摘要
【目的】探讨不同提取方法对测定蜂胶产品中总黄酮含量的影响。【方法】以芦丁为对照品,采用分光光度法测定不同方法所提取的蜂胶中总黄酮的含量。【结果】药典法和甲醇索氏提取法所得总黄酮的含量较高,以芦丁计分别为0.1982、0.1978 mg/g,两者无显著性差异。【结论】与药典法比较,甲醇索氏提取法简便、省时、经济,在测定总黄酮的含量时可以考虑用甲醇索氏提取法来代替药典法。
[ Objective] To explore the effect of different extraction methods on the content of total flavones from propolis. [ Methods ] With rutin as the control, spectmphotometry was used to investigate the content of total flavones extracted from propolis. [ Results ] The content of total flavones extracted by pharmacopoeia extraction method was 0. 1982 mg/g and 0. 1978 mg/g by methanol Soxhlet extraction method, the difference being insignificant. [ Conclusion] Methanol Soxhlet extraction method is simple, effective and practical, and can be used to replace pharmacopoeia extraction method for the determination of total flavones from pmpolis when necessary.
出处
《广州中医药大学学报》
CAS
2007年第3期238-240,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广州市科技计划资助项目(编号:2005-E0101)
关键词
蜂胶/化学
总黄酮/分析
分光光度法
紫外线
PROPOLIS/chemistry
TOTAL FLAVONES/analysis
SPECTROPHOTOMETRY, ULTRAVIOLET