摘要
以猪皮为原料,首先对猪皮胶原蛋白进行高温变性处理,再通过胃蛋白酶降解,用硫代巴比妥酸法(Thiobarbituric acid reactive substances assay,TBA法)评价酶解产物的抗氧化活性,并用SDS-PAGE电泳测定产物的分子量。结果表明,高温处理猪皮5min,胃蛋白酶用量为底物的1.5%,酶解时间为0.5h,酶解产物的抗氧化活性最强。但与抗坏血酸相比,酶解产物没有表现出还原能力。
The hydrolysis of pig skin collagen is carried out with pepsin digestion after denaturalization under high temperature. The antioxidative activity of the hydrolysate is determined with thiobarbituric acid reactive substances assay （TBA）. The molecular weight of hydrolysate is detected with SDS-PAGE. The suitable hydrolysis condition is given as denaturalization time 5 min, ratio of pepsin 1.5%, total hydrolysis time is 0.5 h.
出处
《保鲜与加工》
CAS
2007年第3期28-31,共4页
Storage and Process
关键词
胶原蛋白
酶解
抗氧化
collagen
enzyme hydrolysis
antioxidative
