人工合成九种人类免疫缺陷病毒多肽的抗原性研究

ANTIGENICITY OF NINE SYNTHETIC PEPTIDES OF HUMAN IMMUNODIFICIENCY VIRUS

为了建立敏感、特异的人类免疫缺陷病毒（ＨＩＶ）抗体检测方法，采用固相法合成了ＨＩＶ－１和ＨＩＶ－２的９条多肽，多肽分别位于ＨＩＶｇｐ４１、ｐ２４和ｇｐ３６区域，长度为１０～２７个氨基酸。以合成肽为抗原分别包被酶标板，采用间接ＥＬＩＳＡ法，比较检测了１０份抗－ＨＩＶ－１型和４份抗－ＨＩＶ－２型阳性血清，结果表明ｇｐ４１的１０肽（ＳＰ１）、２１肽（ＳＰ５）、２６肽（ＳＰ６）、２７肽（ＳＰ７）均能检出１００％的抗－ＨＩＶ－１阳性血清，以ＳＰ７抗原性最强。ｇｐ３６的２６肽（ＳＰ８）、２７肽（ＳＰ９）能检出全部的抗－ＨＩＶ－２阳性血清，两者对抗－ＨＩＶ－２血清的亲和性相似，但对抗－ＨＩＶ－１型血清的反应性以ＳＰ８强。以ＳＰ７和ＳＰ８混合包板，能同时检出全部抗－ＨＩＶ－１和抗－ＨＩＶ－２阳性血清；与ＵＢＩ试剂比较，检测了６０份抗－ＨＩＶ阳性血清标本及９６份阴性血清标本，其阳性符合率为９８．３３％（５９／６０），阴性符合率为１００％，总符合率为９９．３６％（１５５／１５６）。表明ＳＰ７和ＳＰ８混合包板可用于ＨＩＶ感染的诊断。

To establish a ELISA method to detect the infection of human immunodificiency virus (HIV), we synthesized nine peptides ranging in length between 10～27 amino acid (aa) of HIV located in the regions of gp41, p24 of HIV 1 and gp36 of HIV 2, according to the published amino acid sequence and the position of antigenic determinants of viruses, by a solid phase method. We detected 10 positive sera of HIV 1 and 4 positive sera of HIV 2 by a indirect ELISA using these synthetic peptides as the coating antigen. The results indicated that all of 10 serum specimens of HIV 1 were positive using SP1, 5, 6, and 7 derived from gp41, and SP7 was the best in antigenicity. The 4 serum specimens of HIV 2 were positive using SP8 and SP9 in the region of gp36 and their antigenicity to HIV 2 were similar, but the antigenicity of SP8 to HIV 1 was higher than that of SP9. All sera of HIV 1 and HIV 2 were positive using SP7 and SP8 as the mixed coating antigen. A comparison of our reagent with UBI reagent in detection of 60 positive and 96 negative control sera for HIV showed that the coincident rate for positive ness was 98.33% (59/60), for negative ness was 100%, and the general coincident rate was 99.36% (155/156). It is concluded that SP7 and SP8 as the mixed coating antigen are applicable in the diagnosis of HIV infection.