电堆集富集非水毛细管电泳同时测定水杨酸、肉桂酸、阿魏酸和香草酸

Separation and Determination of Salicylic Acid,Cinnamic Acid, Ferulic Acid and Vanillic Acid with Sample Stacking-Non-aqueous Electrophoresis

建立了同时分离测定水杨酸、肉桂酸、阿魏酸和香草酸的电堆集富集-非水毛细管电泳（NACE）的新方法。运行缓冲溶液为40mmol/L乙酸钠-2.5mmol/L氢氧化钠甲醇溶液,电压-25kV,在225nm波长下紫外检测。对电压、乙酸钠浓度、氢氧化钠浓度、进样时间、样品溶液等因素对电堆集及分离的影响做了系统的研究。水杨酸、肉桂酸、阿魏酸和香草酸分别在1.4-28mg/L、0.40-8.0mg/L、0.7-18mg/L和0.7-30mg/L范围内线性关系良好（r=0.9999、r=0.9997、r=0.9994、r=0.9997）;回收率分别为95.8-99.6%、96.2-98·2%、95.7-105%和98.9-103%,基于3倍信噪比（S/N=3）,4种有机酸的检出限分别为0.069、0.051、0.107和0.089mg/L。

A new method for the simultaneous determination of salicylic acid, cinnamic acid, ferulic acid and vanillic acid by stacking-non-aquous-capillary electrophoresis has been proposed. The operating buffer was composed of 40 mmol/L sodium acetate - 2.5 mmol/L sodium hydroxide in methanol. The applied voltage was - 25 kV and the UV detection wavelength was 225 nm. The linear ranges of the method were 1.4 - 28 mg/L （r =0.9999） for salicylic acid, 0.40- 8.0 mg/L （r =0. 9997） for cinnamic acid, 0.7- 18 mg/L （r= 0. 9994） for ferulic acid and 0.7 -30 mg/L （r= 0. 9997） for vanillic acid, the recoveries were 95.8% - 99, 6% ,96, 2% - 98.2%, 95.7% - 105%, 98.9% - 103% and detection limits （ S/N = 3 ） of 0. 069, 0. 051, 0. 107 and 0.089 mg/L for 4 organic acids respectively.