蚕豆花粉体外萌发的超微结构观察

Ultrastructure of Vicia Fava Pollen Germination in Vitro

用扫描电镜和透射电镜研究蚕豆花粉体外萌发的超微结构，绝大多数花粉具远近极双孔沟，沟中有一花粉孔．花粉外壁覆盖层呈特异的细皱网纹，外被嗜锇性物质．花粉孔口仅由花粉内壁构成．当花粉萌发时，精细胞胞质、核内可见成束微管，胞内偶见微丝样结构．生长的花粉管顶端含絮状物质小泡和顶端管外细胞壁纤维构成有密切相关．

ultrastructural cvents of Vicia fava pollen germination were studied by SEM and TEM.Under SEM, most of the spheroidal Vicia fava pollengrains have the characteristic of two anacatacolp orate in which the germinal ectoaperture is located. The surface ornamentation of grain posseses special rugulosus coated with osmiumphiled sporopollenin obesrved by TEM. The ectoaperture is covered only with intine. During pollen germination, there are microtuble bundles in the cytoplasm and nucleus of generative cell. Microfilament like structure also occurs in the cytoplasm. There appears to be an close association between the vesicles contained coarse floss material and the fibrillar structure coated in the browing tube tip.