提高桔梗组织培养效率的研究

Study on the Efficiency of the Plant Tissue Culture of Platycodon grandiflorum(Jacp.) A. DC

以MS和N6为基本培养基,配制成不同激素种类和浓度的培养基,以桔梗的叶片、茎段和根段为外植体,探索通过诱导胚状体直接分化出小苗的路子。结果表明:在添加0.5 mg/L NAA和1.0 mg/L 6-BA的MS和N6培养基上,以叶片为外植体时,能诱导胚状体并直接分化出小苗;以茎段为外植体时,部分细胞团通过胚状体直接分化出小苗,另一部分细胞团中长出愈伤组织;以根段为外植体时只长出愈伤组织;在添加不同浓度2,4-D的培养基上只长出愈伤组织,不直接分化出小苗。

In order to increase the efficiency of somatic cell culture of platycodon grandiflorum （Jacp.） A. DC., the direct method of differentiation of young sprout by inducing embryoid was used with the basic MS and N6 culture medium with the different sorts and concentrations of phytohormone and with leaf, stem section and root section as the explant. The results showed that embryoid was induced and the young sprout formed from leaf explant with the basic MS and N6 culture medium with 0.5 mg/L NAA and 1.0 mg/L 6-BA;the young sprout from some stem explants and only callus from root explants. The culture medium with the different concentrations of 2,4-D was for eallns growing but young sprout,