p27^(kip1)和cyclin D1在肾癌中的表达及临床意义

Expression of p^(27kip1) and cyclin D1 in renal cell carcinoma and their clinical significance

目的:研究肾癌组织中p27kip1和cyclinD1蛋白的表达,探讨其与肾癌生物学特征的内在联系。方法:应用免疫组化S-P法,对53例肾癌及6例正常肾组织中p27kip1和cyclinD1蛋白进行检测。结果:在正常肾组织中p27kip1均呈阳性表达,p27kip1阳性表达率在肾癌不同临床分期,不同病理分级中差异显著(G1～G2:70.00%,G3～G4:27.27%,P<0.05;T1～T2:70.83%,T3～T4:20.69%,P<0.05)。cyclin D1在正常肾组织呈阴性表达,cyclinD1阳性表达率在肾癌不同临床分期,不同病理分级中差异显著(G1～G2:51.35%,G3～G4:27.27%,P<0.05;T1～T2:58.33%,T3～T4:20.69%,P<0.05)。结论:p27kip1和cyclinD1蛋白在肾癌中的表达与肿瘤生物学行为密切相关,p27kip1和cyclinD1在肾癌的发生、发展中起重要作用。

Objective： To evaluate the expressions of p27^kip1 and cyclin D1 in renal cell carcinoma（ RCC ）,and to explore the association between the biological characteristics of RCC and the expressions of p27^kip1 and cyclin D1. Methods：The expressions of p27^kip1 and cyclin D1 were detected by immunohistochemical S-P method in 53 cases of RCC and 6 normal renal tissues. Results：In normal renal tissues, p27^kip1 was positively expressed and cyclin D1 was negatively expressed. There were significant differences in the positive expression rates of both p27^kip1 and cyclin D1 in renal cell carcinomas of different pathological grades and clinical stages （p27Kip1：G1 to G2 70.00%,G3 to G4 27.27%,T1 to T2 70.83%,T3 to T4 20.69%;cyclin DI：G1 to G2 51.35%,G3 to G4 27.27%,T1 to T2 58.33%,T3 to T4 20.69%;all P〈 0.05 ）. Conclusion：The expressions of p27^kip1 and cyclin D1 are closely correlated with biological behavior of RCC. p27^kip1 and cyclin D1 may play important roles in the occurrence and development of RCC.