外阴鳞癌MDM2表达与HPV感染状态的关系

Relationship between MDM 2 expression and human papillomavirus status in vulvar squamous cell carcinoma

目的:探讨鼠双微体基因(MDM2)表达与不同人类乳头状瘤病毒(HPV)感染状态的外阴鳞癌发生、发展的关系。方法:采用RT-PCR和Western blotting检测42例外阴鳞癌组织、10例正常外阴组织中MDM2基因mRNA及蛋白表达,PCR检测其HPV-DNA,分析HPV阳性组和阴性组外阴鳞癌组织中MDM2基因mRNA及蛋白表达与其临床病理特征的关系。结果:MDM2基因mRNA在外阴鳞癌组织中相对含量及阳性表达率(0.54±0.35,38.1%)明显高于正常外阴组织(0.21±0.04,0;P<0.05);低分化癌(1.01±0.45,72.7%)与中、高分化癌(0.41±0.18,27.8%;0.32±0.03,23.1%)分别比较,差异有显著性(P<0.05)。MDM2蛋白在外阴鳞癌组织中相对含量及阳性表达率(1.11±0.16,33.3%)明显高于正常外阴组织(0.87±0.02,0;P<0.05);低分化癌(1.27±0.14,63.6%)与中、高分化癌(1.09±0.17,22.2%;1.04±0.12,23.1%)分别比较,差异有显著性(P<0.05)。MDM2基因mRNA及蛋白表达在HPV阳性组与阴性组组间比较,差异无显著性。结论:在外阴鳞癌的形成和发展中MDM2基因与HPV可能作为两个独立的因素起作用;MDM2可能参与了外阴鳞癌的发生发展过程,并可能成为与外阴鳞癌发生发展相关的癌基因。

Objective：To investigate the relationship between MDM2 expression and human papillomavirus （HPV） status in vulvar squa- mous cell carcinomas （VSCC）. Methods：The expressions of MDM2 mRNA,MDM2 protein,and HPV DNA in 42 cases of VSCC and 10 normal vulvar tissues were determined by reverse transcription polymerase chain reaction （RT-PCR）, Western blotting,and PCR,respectively. Results：The relative content and positive expression rate of MDM2 mRNA in VSCC were significantly higher than those in normal vulvar tissues （0.54±0.35 vs. 0.21±0.04,38.1% vs. 0,P 〈 0.05 ）. The relative content and positive expression rate of MDM2 mRNA were 1.01± 0.45 and 72.7%,0.41±0.18 and 27.8%,and 0.32±0.03 and 23.1% in poorly,moderately,and highly differentiated VSCC,respectively,and there were significant differences between poorly differentiated VSCC and moderately and highly differentiated VSCC （P 〈 0.05 ）. The relative content and positive expression rate of MDM2 protein in VSCC were significantly higher than those in normal vulvar tissues （1.11±0.16 vs. 0.87±0.02,33.3% vs. 0,P 〈 0.05 ）. The relative content and positive expression rate of MDM2 protein were 1.27±0.14 and 63.6%, 1.09±0.17 and 22.2%,and 1.04±0.12 and 23.1% in poorly,moderately,and highly differentiated VSCC,respectively,and there were significant differences between pcody differentiated VSCC and moderately and highly differentiated VSCC （P 〈 0.05）. No significant difference was found in the expressions of MDM2 mRNA and protein between HPV-positive group and HPV-negative group. Conclusion：MDM2 and HPV may be 2 independent factors during the formation and development of VSCC. MDM2 is involved in the occurrence and development of VSCC.