抗药性链霉菌S89的诱变选育及对灰霉病菌的抑制作用

Mutation breeding of resistant streptomyces strain S89 and its inhibiting effect on Botrytis cinerea

采用紫外线照射与药物培养基驯化相结合的方法选育抗药性链霉菌,筛选出4株对腐霉利有显著抗性的突变株(US-1,US-2,US-3和US-4)。US-1和US-3对灰葡萄孢菌的抑菌能力显著高于亲本菌株,US-2和US-4的抑菌能力显著低于亲本菌株。颉颃活性最强的US-1菌株与腐霉利的协同作用表明,7份菌液和3份药液混用后,对灰葡萄孢菌的抑菌圈直径为21.97mm,对离体番茄灰霉病的防效高达77.68%,显著高于菌株单独使用的抑菌效果。

4 mutation strains resistant to procymidone, namely, US-1, US-2, US-3, US-4, were screened out through the combination method of UV irradiation and medical cultivation. Inhibition activities of US-1 and US-3 were more significant than their parental strains, inhibition activities of US-2 and US-4 were significantly lower than that of parental strains. Co-action of US-1 whose inhibition activity was strongest and procymidone to botrytis cinerea was studied. Results showed that when 7 shares of US-1 mixed with 3 shares of procymidone its diameter of inhibition zone was 21.97mm, its control effect in vitro on Botrytis cinerea reached 77.68%, obviously more than that of US-1 or procymidone alone.