HPLC法测定人血浆中苯溴马隆浓度

Determination the Concentration of Benzbromarone in Human Plasma by HPLC

目的:建立人血浆中苯溴马隆浓度的HPLC测定方法。方法:色谱柱为Hypersil BDS C_(18)(250 mm×4.6 mm,5μm);流动相为甲醇.水.三乙胺(48:52:0.02),流速1ml·min^(-1);紫外检测波长357 nm;以香豆素为内标,采用乙腈直接沉淀蛋白后进样。结果:苯溴马隆的线性范围为0.01～0.5 mg·L^(-1)(r=0.999 2);提取回收率为103.5%,106.8%,方法回收率为109.5～113.2%,日内和日间RSD分别小于7.0%和9.5%结论:本法操作简便、灵敏、准确,可用于临床治疗度高。

Objective： To develop a method for the determination of Benzbromarone concentration in human plasma by HPLC. Method： The Hypersil BDS C,s column（250 mm× 4.6 mm, 5μm）was used. The mobile phase was methanol-water- triethylamine（48 ：52： 0.02） and flow rate was 1 ml·min^-1. The detecting wavelength was at 357 nm. Cumarin was used as the internal standard. The sample was deposited by aeetonitrile and then injeetied. Result： The linear range of benzbromarone was 103.5% - 106.8%, The method recovery was 0.01 - 0.5 mg· L^-1 （ r = 0.999 9）, the extration recovery was 109.5 - 113.2%, the with in day less than 7.0% and between day lessthan 9.5%. Conclusion： The method is simple, sensitive and accurate which is sumitable for clinic therapeutic drug monitoring.