HPLC法测定红药片中三七皂苷R_1和人参皂苷Rg_1的含量

Determination the Content of Notoginsenoside R_1 and Ginsenoside Rg_1 in Hongyao Tablets by HPLC

目的:建立红药片的HPLC含量测定方法。方法:色谱柱:Agilent Extend-C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-0.3%磷酸溶液(19:81),流速:1.0ml·min^(-1),检测波长:203 nm,柱温:40℃。结果:三七皂苷R_1在0.03～0.90μg范围内线性关系良好(r=0.999 9);平均加样回收率为100.0%,RSD=1.3%(n=5);人参皂苷Rg_1在0.10～3.00μg范围内线性关系良好(r=1.000 0);平均加样回收率为99.5%,RSD=0.6%(n=5)。结论:该方法简便易行,结果准确,可适用于红药片的质量控制。

Objective： To establish a HPLC method for determination the content of notoginsenoside R1 and ginsenoside Rg1 in Hongyao tablets. Method： The agilent extend C18 column（ 250 mm× 4.6 mm ,5μm）was used, the acetonitrile-0.3% phosphoric acid solution（ 19：81 ） as mobile phase, the flow rate was 1.0 ml·min^-1, The detection wavelength was 203 nm, the column temperature was 40℃. Result： The linear ranges of notoginsenoside R1 and ginsenoside Rg1 were at 0.03 - 0.90μg（ r = 0.999 9 ） and 0.10 - 3.00μg（ r = 1. 000 0） respectively, The average recoveries（ n = 5 ） were 100.0% and 99.5% with RSD 1.3% and 0.6% respectively. Conclusion： The method is simple and accurate. It can be used for quality control of hongyao tablets.