HPLC法测定利胆消石丸中黄芩苷的含量

Determination of Baicalin in Lidan Xiaoshi Pills by HPLC

目的:建立HPLC法测定利胆消石丸中黄芩苷含量的方法。方法:采用Shim-pack VP-ODS色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(47:53:0.2),检测波长280nm,流速1.0ml·min^(-1),柱温加℃。结果:黄芩苷在0.24～1.21μg(r=0.999 9)范围内呈良好的线性关系;平均加样回收率为101.1%,RSD=2.0%(n=6)。结论:该方法简便,准确,可用于利胆消石丸的质量控制。

Objective： To establish a method for determination the content of baicalin in Lidan Xiaoshi Pills by HPLC. Method： Shim-pack VP-ODS column （150 mm × 4.6 mm, 5 μm） was used;The mobile phase was methanol-water-phosphoric acid（47： 53： 0.2） with the flow rate was 1.0 ml·min^-1 ;The detection wavelength was at 280 nm and the column temperature was 40℃. Result： The linear range of baicalin was 0.24 - 1.21 μg （r= 0. 999 9 ）. The average recovery was 101.1%, RSD = 2.0% （ n = 6）. Conclusion; The method is simple and accurate. It can be used for the quality control of Lidan Xiaoshi Pills.