伴刀豆球蛋白A/葡聚糖修饰的金纳米颗粒自组装膜增强信号的表面等离子体共振葡萄糖传感器

A signal enhanced glucose surface plasmon resonance sensor based on concanavalin A /dextran modified Au nanoparticles self-assembled bilayer

通过特异性识别作用在表面等离子体共振传感器的金膜表面构建了伴刀豆球蛋白A/葡聚糖修饰的金纳米颗粒自组装膜。当有葡萄糖存在时,膜被分解,从而实现对葡萄糖的灵敏检测。结果表明:由于金纳米颗粒和金膜之间的等离子体波耦合作用,修饰了金纳米颗粒的自组装膜上,葡萄糖的检测信号有明显增强。该传感器可以选择性地检测0.1～100mmol/L浓度范围内的葡萄糖溶液,且敏感膜可以多次再生使用。

The concanavalin A / dextran - modified Au nanoparticles self - assembled bilayer was built on Au film through biospecific interaction. At the presence of glucose, the self - assembled bilayer was disintegrated and the surface plasmon resonance signal changed. Hence, the glucose concentrations were detected. The results showed that the dextran - AuNPs was effective for enhancing the signal changes due to the coupling of plasmon wave between AuNPs and Au films. This sensor could detect the glucose selectively in the range of 0, 1 - 100 mmol/L, and the sensing element could be regenerated for several times.