磷细菌PB12菌株的选育及培养条件的研究

Breeding and Culture Conditions of Phosphate Dissolving Bacterium PB12

以PB-1P为出发菌株,经紫外线诱变,获得1株解磷高产菌株PB12。在以磷酸三钙为惟一磷源的培养基中接种PB12菌株,28℃,振荡培养5d,培养基上清液中磷含量达61.21mg/L,是出发菌株的2.03倍。同时研究了各种理化因素对PB12菌株生长的影响,确定了最佳培养条件,即玉米粉1.0%,豆饼粉2.0%,pH7.0,装液量为100mL(500mL三角瓶),36℃培养42h,有效活菌数可达10.2×108cfu/mL。

PB12 with strong ability of dissolving phosphate was obtained from PB-1P through UV mutagenesis. It was cultured in shaking flask containing medium with Ca3 （PO4 ）2 as the only phosphorous source, five days after shaking culture under 25 ℃ , phosphorous content in the supernatant was 61.21 mg/L 2.03 times that of the original strain. At the same time, influence of different physical and chemical factors on the growth of PB12 were also studied to confirm the optimum culture conditions, they are 1.0% maize powder, 2.0% soybean powder, pH 7.0, filling amount 100 mL （in 500 mL flask） , culture under 36 ℃ for 42 hours, the effectively live bacteria could reach up to 10.2 -10s cfu/mL.