摘要
目的本文将1996年3月至2005年5月门诊及住院儿童年龄0～6岁2374人，0～1岁1573人，1～3岁542人，〉3岁259人进行骨碱性磷酸酶测定（BALP）。探讨了随着佝偻病防治工作的不断深入，典型佝偻病已明显减少，而处于-3P-临床状态的疑似病人逐渐增多，因此对维生素D缺乏的亚临床病儿及时早期诊断和治疗，才能降低佝偻病的发病率。方法采用中国科学院协和试剂开发部提供的试剂盒，用全血干化学免疫浓缩方法，采末梢血30μl，加入试剂孔中，37℃孵育染色6分钟，对照标准色板判断骨碱性磷酸酶活性。结果BALP异常1686人占71．8％，亚临床型佝偻病928人，占39％，初期佝偻病564人，占24％，激期194人，占8．2％。结论BALP是反应骨生长障碍最敏感、最特异的指标之一，对亚临床佝偻病的早期诊断具有很大的实用价值。
Objective Determining the concentration of SALD in children either out patientor in hospital since Mar 1996 to May 2005. Age： 0 -6 years 2514 cases; 0 - 1 years, 1573 cases; 1 -3 years,542cases; 3 -6years,259cases; To study with the continual progress of ra- chitis prevention and cure typical rachitis has been reduced obviously. Equivocal patients standing in the subclinical position are gradually increasingl, so, oncy diagnosing and curing subclinical patients standing in the subclinical and curing subclinical patients who lack VD in ther body prictineiy, Cun it lesseb the disease incidence of rachitis. Methods Adopting the kit provided by XIHE agentiu exploitation deprtment of Academia Sinica. Using chemi - immune concentration method, drawing 301xl peripheral blood and adding it into agetiu gabto be incubated and stained for 6min at the tempoerature of 37℃. Theniudgeing the activity of BALP though comparing standard shade guide. Result There are 1686 cases, 71.8% in total, manifest alnormal BACP pactivity;there are 928 cases, 39% in total, are in subclinical position; 564 cases, 24% in total, stand in initial stage; 194 cases, 8.2% in total, stand in active stage. Conclusion BALP is one of the most sensitive and specific index which reflect the hypotrophy of bone. It has extremely gragmatie value for the subclinial rachitis early diagnosis.
