弓形虫自然弱毒株棒状蛋白2基因克隆及序列分析

Cloning and analysis of nucleotide sequences encoding rhoptry protein2 of Toxoplasma gondii

应用PCR法从弓形虫QHO株速殖子基因组DNA中扩增ROP2的基因片断,连接到pMD18-T载体中,转化大肠杆菌JM109,经酶切及PCR鉴定后进行测序,并进行序列分析.结果表明,自然弱毒株的开放阅读框架(ORF)与ROP2基因已知序列(Z36906)的ORF具有97%的同源性,氨基酸序列具有95%的同源性.

The gene coding for rhoptry protein2 （ROP2） from the total DNA of Toxoplasma gondii of QHO strain was cloned and analyzed in the study. The gene fragment encoding ROP2 was amplified by PCR from the total DNA of Toxoplasma gondii of QHO strain. And the gene of RQP2 was subeloned into the plasmid pMD18-T. The constructed recombinant plasmid pMD18-T-ROP2 was transferred into E. coli competent cell JM109. After identification by PCR and restriction enzyme eleavage,DNA sequence was determined. Homology analysis showed that the rhoptry protein2 sequences of QHO strain shared 97 homology with that of RH strain,and the deduced amino acid sequences from rhoptry protein 2 sequences of QHO strain shared 95 % homology with that from rhoptry protein 2 sequences of RH strain.