大鼠视网膜缺血再灌注后HIF-1α的表达与视网膜细胞凋亡的研究(英文)

HIF-1α expression and retinal cell apoptosis in rat retina ischemia-reperfusion injury

目的:探讨大鼠视网膜细胞缺血再灌注后低氧诱导因子(HIF-1α)的表达、视网膜细胞凋亡的发生以及二者之间的关系。方法:采用前房灌注生理盐水升高大鼠眼压到110mmHg(1kPa=7.5mmHg)持续1h的方法制作实验性视网膜缺血再灌注损伤模型,分别于再灌注不同时间点取材,行免疫组织化学法染色观察HIF-1α在视网膜细胞的阳性表达;采用DNA原位末端标记(terminal dUTP nick end labelling,TUNEL)法定位凋亡的视网膜细胞来检测视网膜凋亡细胞。结果:缺血再灌注2h组视网膜神经节细胞层及内核层细胞出现HIF-1α弱阳性表达,12h组阳性表达至高峰,24h组HIF-1α表达渐减少。视网膜组织细胞凋亡见于缺血再灌注12,24及48h组,凋亡细胞主要位于内核层,且24h组凋亡阳性表达最强。结论:缺血再灌注后大鼠视网膜HIF-1α的表达增加,参与视网膜缺血再灌注损伤;视网膜细胞的损伤部分以凋亡的形式发生,HIF-1α的表达可能与视网膜细胞凋亡有密切的关系。

AIM：Investigating the expression of HIF-1α, apoptosis of retinal cells and the role of HIF-1α in apoptosis in rats＇ retinal ischemia-reperfusion injury. METHODS：The rat model of experimental retinal ischemia-reperfusion injury was established by increasing the intraocular pressure to 110mmHg （1kPa =7.5mmHg） in rat eyes. At different time points of post ischemia, the expression of HIF-1α of the retina was detected by immunohistochemical staining, and apoptosis of the retinal cell was detected by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphatebiotin nick end labeling （TUNEL）. RESULTS： HIF-1α appeared in the cells of retinal ganglion layer and inner nuclear layer at 2 hours after ischemia .The expression reached to a peak, 12 hours after retinal ischemia-reperfusion, then the expression was declined. The apoptotic cells were mainly in inner nuclear layer and could be detected at the 12th, 24th and 48th hour after ischemia, the peak value was the group of 24th hour. CONCLUSION： Expression of HIF-1α in the rats＇ retina is greatly enhanced after ischemia-reperfusion, which may be involved in the retinal injury; the injury of retinal neurons occurs partly in the form of apoptosis. The expression of HIF-1α may play an important role in cell apoptosis.