摘要
目的:观察自体TGFβ2-DC对于同种异体角膜移植术后免疫排斥反应的调节作用。方法:PBS或已摄取供体抗原的自体TGFβ2-DC经小鼠尾静脉注射7d后,建立小鼠同种异体角膜移植模型,临床观察两组植片混浊程度等情况,比较植片存活时间。结果:TGFβ2-DC注射后,植片发生移植排斥反应的时间延迟,植片存活时间明显延长,PBS组及TGFβ2-DC尾静脉组植片的存活时间分别为17.8±3.01d和37.6±1.65d(P<0.01)。结论:摄取供体抗原的不成熟自体DC可以诱导受体获得供体抗原的特异性免疫耐受,使小鼠同种异体角膜移植的免疫排斥反应延迟,植片存活时间明显延长。
AIM： To observe the suppression effect of syngeneic TGFβ2-DC on corneal allograft rejection.
METHODS： PBS or TGFβ2-DC incubated with donor antigen was injected into murine tail vein. Seven days after the injection. the corneal allograft transplantations were performed and eyes were evaluated by clinical observation.
RESULTS： After TGFβ2-DC injection, the corneal allograft rejection was delayed and the survival time of grafts was prolonged. The mean survival time of PBS injection group and TGFβ2-DC injection group was 17.8±3.01d and 37.6±1.65d, respectively （P〈 0.001）.
CONCLUSION： The immature syngeneic TGFβ2-DC captured with donor antigen can induce donor-specific immune tolerance in the recipients, in which the corneal allograft rejection is delayed and the allograft survival time is prolonged.
出处
《国际眼科杂志》
CAS
2007年第2期346-349,共4页
International Eye Science
基金
中国国家自然科学基金资助项目(No.30500550)~~
