摘要
目的:探讨脂质体介导的TGF-β1基因(pEGFP-TGF-β1)转染对人晶状体上皮细胞(HLEC)系B3(HLEC-B3)增殖、凋亡及细胞周期的影响。方法:将pEGFP-TGF-β1转染HLEC-B3,观察细胞形态变化;采用生长曲线法、噻唑蓝比色法分析细胞增殖改变;使用流式细胞仪测定细胞凋亡变化、分析细胞周期改变(DNA倍体法)。结果:pEGFP-TGF-β1成功转染后,HLEC-B3逐渐变圆、脱壁。转染后24~48h细胞的增殖受到抑制,与相应对照组比较,差异均有统计学意义(P<0.01);凋亡细胞比例(转染24h为(21.0±1.7)%,转染48h至(43.6±1.4)%明显升高,与相应对照组[24h为(0.42±0.06)%,48h为(0.60±0.02%)]比较,差异均有统计学意义(P<0.01);细胞出现G1期阻滞现象,表现为G1期细胞比例[转染24h为(72.0±1.9)%,转染48h为(74.7±2.2)%]增加和S期细胞比例[转染24h为(20.4±2.2)%,转染48h为(19.4±1.4)%]下降,与相应对照组比较,差异均有统计学意义(P<0.01)。结论:pEGFP-TGF-β1可成功转染HLEC-B3,诱发细胞G1期阻滞,有效抑制细胞增殖,诱导细胞凋亡。
AIM:To investigate the effects of pEGFP-TGF-β1 plasmid DNA on proliferation and survival of human lens epithelial cell.
METHODS:Immortalized human lens epithelial cell line (HLEC- B3) was transfected by pEGFP-TGF-β1 genes, respectively. Cell proliferation was analyzed by cell growth curve and MTT celorimetric assay, cell apoptosis and cell cycle of HLEC-B3 were examined by flowcytometry.
RESULTS:HLEC-B3 cells were transfected successfully, pEGFP-TGF-β1, suppressed the growth rate, enhanced the cell apoptosis(21.0±1.7) % after 24 hours and (43.6±1.4)% after 48 hours transfection. The proportions of G1 phase cells were increased to (72.0±1.9)% after 24 hours and (74.7±2.2)% after 48 hours transfection, while the proportions of S phase cells were decreased to(20.5±2.2)% after 24 hours and (19.4± 1.4)% after 48 hours transfection.
CONCLUSION: pEGFP-TGF-β1 genes can successfully trans- feet HLEC-B3, inhibit proliferation and induce apoptosis of HLEC-B3.
出处
《国际眼科杂志》
CAS
2007年第2期357-360,共4页
International Eye Science