摘要
目的:探讨高糖培养牛视网膜微血管周细胞FOXO1A表达。方法:原代培养周细胞,用免疫细胞化学和Westernblot方法检测高糖诱导的牛视网膜微血管周细胞FOXO1A蛋白表达。结果:视网膜微血管周细胞在5mmol/L D-葡萄糖培养72h后,FOXO1A免疫反应性主要表现在细胞核,在30mmol/L D-葡萄糖或5μ mol/LH2O2培养72h后,FOX-O1A免疫反应性显着增加,FOXO1A免疫反应性主要表现在细胞核和细胞质;Western blot发现高糖组和过氧化氢组周细胞培养48,72h后FOXO1A蛋白活性和表达较对照组显着增加。结论:高糖诱导的视网膜微血管周细胞FOXO1A表达和活性显著增加。
AIM： To study the expression of FOXOIA in bovine retinal microvascular pericytes cultured with the high glucose medium.
METHODS： Primary bovine retinal microvascular pericytes were cultured. FOXOIA expression was measured in bovine retinal microvascular pericytes treated with low glucose （5 mmol/L）, high glucose （30mmol/L） and H2O2 （5μmol/L） in medium containing 20mL/L fetal bovine serum using immunocytochemistry and Western Blot analysis.
RESULTS： Slight FOXOIA immunoreactivity was detected the retinal microvascular pericytes treated with low glucose after 72 hours distributed in the nucleus. FOXOIA immunoreactivity was significantly increased in the retinal microvascular pericytas treated with high glucose and H2O2 after 72 hours and distributed in the nucleus and cytoplasm of pericytes. Western Blot showed that FOXOIA protein expression levels increased in high glucose and H2O2 groups after 48 hours, 72 hours compared with that in low high group and phos- phor-FOXO1A protein expression levels reduced after 48 hours, 72 hours compared with that in low high group.
CONCLUSION： These data show that high glucose induced the over-expression of FOXOIA in the retinal microvascular pericytas.
出处
《国际眼科杂志》
CAS
2007年第2期373-376,共4页
International Eye Science
