人腺样囊性癌多药耐药细胞系的建立

Establishment of multidrug cell line of human adenoid cystic carcinoma

目的:建立人腺样囊性癌耐药细胞系,为阐明腺样囊性癌的多药耐药机制及逆转耐药提供模型及研究依据。方法:以长春新碱(VCR)为诱导剂,通过浓度递增间断刺激法对人腺样囊性癌细胞系(ACC)进行体外诱导耐药,建立耐VCR的腺样囊性癌细胞系ACC/VCR。细胞计数法绘制细胞生长曲线,噻唑蓝(MTT)比色法检测细胞对化疗药物敏感性。结果:ACC经体外诱导后,ACC/VCR细胞对长春新碱(VCR)、阿霉素(ADM)和平阳霉素(PYM)的耐药性明显增强,具有交叉耐药,对环磷酰胺(CTX)、氟尿嘧啶(5-FU)和顺铂(DDP)耐药性无明显变化。耐药前后ACC细胞的生长曲线、群体倍增时间和光镜下的形态无明显改变。结论:VCR可诱导腺样囊性癌细胞产生多药耐药。

AIM： To establish an in vitro resistance model of adenoid cystic carcinoma which can provide theoretical information for elucidating the mechanisms of multidrug resistance （MDR）, improving the chemotherapy scheme, evaluating the prognosis and reserving the MDR in adenoid cystic carcinoma. METHODS： The resistance cell line, ACC/VCR, was induced in the ACC cell line in vitro by progressive concentrations of vincristine （VCR）, a drug of choice in the treatment of adenoid cystic carcinoma. The growth curve, drug sensitivity in the parental and resistant cell lines were investigated by cell counting and MTT assay. RESULTS： ACC/VCR cell line was cross-resistant to vincristine （VCR）, adriamycine （ADM） and pingyangmycin （PYM）, but not resistant to Cyclophosphamide （CTX）, fluorouracil （5-FU） and dsplatin （DDP）. There was no significant difference in the doubling time and morphological changes betwean AOC cell line and ACC/VCR cell line. CONCLUSION： Exposing to VCR can induce MDR in adenoid cystic carcinoma.