金黄地鼠胰岛素抵抗和糖尿病动物模型的建立

Establishment of an Insulin-Resistant and Type 2 Diabetic Model in Golden Hamster

目的建立胰岛素抵抗和糖尿病动物模型。方法给金黄地鼠喂以高脂果糖饲料,部分给小剂量链脲菌素(30mg/kg)腹腔注射,以正常金黄地鼠作为对照,测定动物体重、空腹血糖、血脂、胰岛素水平,计算胰岛素敏感指数,并对肝脏、胰腺及主动脉弓组织进行形态学比较。结果金黄地鼠经高脂果糖喂养6周制成胰岛素抵抗、肥胖和脂质代谢紊乱的动物模型。再经小剂量一次性腹腔注射链脲菌素后,约80%的胰岛素抵抗和肥胖的金黄地鼠出现显性糖尿病。结论高脂果糖饲料结合小剂量链脲菌素腹腔注射可以制成胰岛素抵抗的2型糖尿病金黄地鼠模型。

Objective To establish an experimental animal model with insulin resistance and/or type 2 diabetes mellitus. Methods High energy fructose diet was given to mesocricetus to produce insulin resistance and hyperlipidemic models. Diabetes was induced by a single intraperitoneal injection of streptozotocin （30 mg/kg） on these insulin resistant animals. Normal animals were used as control. Serum glucose, hpid profiles and insulin levels were measured to verify the diabetic and hyperlipidemic models. The liver, pancreas and aortic arch were examined to distinguish the histological difference among the animals of three groups. Results The mesocricetus fed with high energy fructose diet for 6 weeks developed insulin resistance, obesity and dislipidemia, 80% of which became type 2 diabetic after single intraperitoneal injection of streptozotocin. Conclusion Insulin resistant and diabetic mesocricetus models can be produced successfully with high energy fructose diet and single intraperitoneal injection of streptozotocin.