四种消毒剂对小鼠肝炎病毒MHV-A59的杀灭效果

Evaluation of Inactivation Efficacy of Four Disinfectants against Mouse Hepatitis Virus

目的研究过氧乙酸等4种常规消毒剂对小鼠肝炎病毒MHV-A59的杀灭效果。方法按照2002版《消毒技术规范》中规定的试验方法进行中和剂鉴定试验以及病毒灭活试验。结果与病毒作用5 min和10 min后,3%的过氧乙酸、250 mg/L的碘伏、含有效氯25 mg/L的复方二氯异氰尿酸钠对MHV-A59的杀灭对数值均≥4,具备杀灭作用,而新洁尔灭的杀灭对数值则<4,杀灭能力较低,对该病毒的灭活效果很难达到规范中的要求。结论过氧乙酸、碘伏以及复方二氯异氰尿酸钠对小鼠肝炎病毒的杀灭效果确实,适用于对小鼠肝炎病毒的消毒。

Objective To evaluate the efficacy for 4 disinfectants in inactivation of mouse hepatitis virus （MHV）. Methods According to the ＇Disinfectants Technical Guidelines＇, 2002 ed, neutralization and MHV inactivation tests were used to evaluate their antiviral activity. Results After treatment with MHV-A59 for 5 min and 10 min, the inactivation logarithmic value of peracetic acid disinfectant, iodophor, sodium dichloroisocyanurate were higher than 4. The inactivation logarithmic value of benzalkonium bromide was lower than 4, indicating a low inactivation efficacy, which could not satisfy the requirements of guidelines. Conclusion Peracetic acid disinfectant, iodophor and sodium dichloroisocyanurate have good efficacy in inactivation of MHV-A59.