秦川牛GHR基因SNPs及其与生长性状关系的研究

Study on SNPs of GHR gene and its associations with growth traits in Qinchuan cattle

利用PCR-SSCP技术研究了136头秦川牛GHR基因第10外显子多态性,所研究的秦川牛群体GHR基因该座位存在多态性,发现了A、B、C3个等位基因,其基因频率依次为0.5956、0.2905、0.1140,并且群体处于Hardy-Weinberg平衡状态。对纯合基因型个体进行测序,测序结果,该座位存在两个突变位点。通过构建最小二乘线性模型,分析了生长激素受体基因与生长性状的关系,表明生长激素受体基因的AB基因型效应在部分生长性状上高于其他基因型,差异达到显著水平,提示GHR基因有可能作为秦川牛生长性状的侯选基因。

In this paper PCR-SSCP technique was used to analyze the polymorphisms of the tenth exon of GHR gene in Qinchuan cattle. The results showed that PCR products demonstrated polymorphisms in Qinchuan cattle and the population was at Hardy-Weinberg equilibrium （P 〉 0.05）. There were three allele gene A , B , C in this locus with the corresponding frequencies of 0.5956, 0.2905 and 0.1140. The amplified fragments of homozygosis genotype were sequenced. The result showed that there were two mutation in these fragments. Then the relationship between genotypes and productive perform- ances was analyzed by the Least Squire Method （LSM）. The results showed that some productive performances of the indi- viduals with genotype AB were higher than those of the other genotypes, and the derivation was significant among geno- types. It suggested that GHR gene may be a candidate gene responsible for growth trait in Qinchuan cattle.