膀胱癌中缺氧诱导因子-1α的表达和肿瘤血管生成的关系

HIF-1α expression correlates with angiogenesis in bladder cancer

目的:探讨缺氧诱导因子-1α(HIF-1α)在膀胱移行细胞癌中的表达和肿瘤血管生成的关系。方法:采用免疫组化SABC法观察42例膀胱移行细胞癌石蜡标本中HIF-1α、VEGF、PCNA的表达情况及微血管密度,并结合临床资料进行分析。结果:HIF-1α在膀胱移行细胞癌中的表达显著增强(P<0.05)并与VEGF、MVD、PCNA和肿瘤复发呈正相关(依次为:P<0.01,P<0.05,P<0.01,P<0.05),但与膀胱癌的病理分级、临床分期和患者年龄无关;且VEGF与MVD在膀胱癌组织中表达增高,与其病理分级相关(P<0.05)。在logistic回归模型中,HIF-1α和PCNA均呈现为膀胱癌复发的危险因素。结论:HIF-1α在膀胱移行细胞癌肿瘤血管生成中起重要作用,并可能成为膀胱癌诊断和复发预测的生物学标记,以及抗血管生成治疗新的靶点。

Objective：To evaluate the expression of HIF-1α in transitional cell carcinoma of the bladder（TCC） and its correlation with angiogenesis and clinical value. Methods..Immunohistochemistry, SABC, was used to detect the expression of HIF-1α, VEGF, CD34 and PCNA in 42 TCC paraffin-embedded tissue samples. The results were analyzed with the clinical data. Results： HIF-1α was obviously higher expressed in TCC than normal tissue. The significant positive associations between HIF-1α and VEGF, MVD, PCNA, and tumor recurrence were found （依次为： P 〈0.01, P 〈0.05, P 〈0.01, P 〈0.05）. But no correlation was found between HIF-1α and tumor stage and grade, The angiogenesis markers were closely related to tumor grade （ P 〈0.05）. In the logistic regression study, HIF-1α and PCNA were clarified as the hazard factors to tumor recurrence. Conclusions： HIF-1α plays an important role in TCC angiogenesis, and could be a new biomarker for TCC diagnosis and recurrence evaluation. It can also be expected to be a new target for antiangiogenesis.