大鼠胰岛细胞转染Wee1Hu基因延长异种胰岛细胞移植后存活时间

Role of Wee1Hu gene in xeno-islet transplantion in diabetic rats

目的 探讨大鼠胰岛细胞转染Wee1Hu基因对异种胰岛细胞移植后存活时间的影响。方法 以Wistar大鼠为供者，Balb／c糖尿病小鼠为受者，进行异种胰岛细胞移植。实验分为2组，每组20只。实验组：将转染Wee1Hu基因的1×10^6个供者胰岛细胞悬于0．5ml无菌生理盐水中，注射至受者的腹腔；空白对照组：将1×10^6个空载体胰岛细胞用与实验组相同的方法注射至受者腹腔。实验组和空白对照组的部分受者在移植前1周腹腔注射降植烷0．5ml，并于胰岛细胞移植后开始口服环孢素A30mg／kg，直至实验结束。监测2组胰岛细胞移植后的胰岛素释放功能及存活时间。结果 胰岛细胞移植后，空白对照组的受者均维持高血糖，且体重持续下降，平均生存时间为（18±2．5）d；实验组的受者血糖在2d内均降至正常，且体重增加，生存时间延长（38±3．5）d；两组相比较，差异有统计学意义（P〈0．05）。空白对照组维持正常血糖时间为（8±2．3）d，而实验组为（10±2．5）d。实验组中采用免疫抑制剂治疗的受者，长期维持正常血糖，平均维持时间超过30d，与空白对照组中采用免疫抑制剂治疗的受者比较，差异有统计学意义（P〈0．05）。结论 大鼠胰岛细胞转染Wee1Hu基因可延长异种胰岛细胞移植后的存活时间，与免疫抑制剂协同作用时效果更佳。

Objective Weel Hu gene modified rat islet cells were transplanted into STZ-diabetes mice to identify effects of Weel Hu gene on the life span and the protective effect on graft. Method The isolated rat islet cells transfected with WeelHu gene were injected intro abdominal STZ-induced diabetes mice to perform islet transplantation with or without immunosuppressive drugs. Results In the diabetic mice transplanted with Weel Hu gene modified rat islet cells without immunosuppressive drugs, the level of blood glucose was dropped quickly to normal and the body weight increased with longer life span. As compared with control group, these data showed significant difference （P〈0.05）. If the mice implanted with Weel Hu gene modified rat islets were subjected to the immunosup pressive drugs, the blood glucose kept normal for a remarkable longer time （P〈0. 05） in comparison with group using immunosuppressive drugs only or group using Weel Hu gene modified rat islet cells only. Conclusion Weel Hu gene can prolong the life-span of graft in xeno-islet transplantation and has a synergistic effect with immunosuppressive drugs.