摘要
目的探讨老年人甘露糖结合凝集素(MBL)基因ExonⅠ52、54和57位密码子点突变频率和血浆MBL含量变化。方法采用PCR-SSP法检测MBL基因ExonⅠ52位密码子点突变,PCR-RFLP法检测54和57位密码子点突变;ELISA法测定血浆MBL含量。结果老年组和中青年组52、54和57位密码子基因突变频率分别为0.6%和0.8%(确切概率P=0.801)(52位)、20.9%和16.4%(P=0.336)(54位)、0%和0%(57位)。血浆MBL含量老年组和中青年组分别为(2017±1804)μg/L和(2523±1955)μg/L(P=0.109)(野生型+突变型组);(2956±1701)μg/L和(3432±1687)μg/L(P=0.1775)(野生型组);(530±360)μg/L和(709±416)μg/L(P=0.3448)(突变型组)。结论MBL基因ExonⅠ52、54、57位密码子点突变频率和MBL血浆含量老年组和中青年组差别无统计学意义。
Objective To investigate point mutation frequency at codon 52, 54 and 57 of mannose-binding lectin (MBL) gene Exon Ⅰ and plasma MBL level. Methods The point mutation at eodon 52 was detected with polymerase chain reaction (PCR) -sequence specific primers (SSP) , that at eodon 54 and 57 of MBL gene Exon Ⅰ was measured by PCR-restrietion fragment length polymorphism (RFLP) ; plasma MBL concentration was determined by ELISA. Results Mutation frequencies at eodon in old age group and middle-aged and young group were O. 6% and O. 8% ( accurate probability P =0. 801 ) ( at 52) , 20. 9% and 16.4% ( P =0. 336) ( at 54), 0% and 0% ( at 57 ). Plasma MBL concentrations in old aged and middle-aged and young group were (2 017 ± 1 804) μg/L and (2 523 ±1 955 ) μg/L ( P = 0. 109 0) ( wild + mutation type), (2 956 ± 1 701 ) μg/L and (3 432 ± 1 687) μg/L ( P =0. 177 5) (wild type), (530 ±2360) μg/L and (709 ±2416)μg/L (P = 0. 344 8) (mutation type). Conclusiolis There are no statistical significance in point mutation frequency at eodon 52, 54 and 57 of MBL gene Exon I between the( old aged group and the middle-aged and young group.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2007年第8期750-752,共3页
Chinese Journal of Gerontology
基金
河北省自然科学基金资助项目(C2006000875)
关键词
甘露糖结合凝集素
PCR—SSP
PCR—RFLP
突变频率
Mannose-binding leetin (MBL)
Polymerase chain reaction (PCR) -sequence specific primers (SSP)
PCR-restrietion fragment length polymorphism (RFLP)
Mutation frequency