人眼结膜上皮干细胞的定位分析

The location of human conjunctival epithelium stem cells

目的探讨人眼结膜上皮干细胞的定位。方法对人眼不同部位(穹隆部、球结膜及睑结膜)结膜上皮组织用组织块培养法,种植于培养皿,观察结膜上皮细胞的生长特性。结果细胞生长速度不同:同一组织块其四周细胞生长速度不同,有的部位细胞生长快,有的部位细胞仅有移行现象;取自同一部位如穹隆部结膜的不同组织块,上皮细胞生长速度也不同,有的组织块周围细胞生长快,有的组织块周围细胞生长慢;但取自穹隆部结膜、睑结膜、角巩缘处球结膜的组织块,细胞生长形态相似。结论结膜干细胞散在分布于穹隆部和睑球结膜,但不均匀,即使在相同部位结膜干细胞分布也不平衡。

Objective To investigate the location of human conjunctival epithelium stem cells. Methods The human conjunctival epithelium tissues got from palpebral conjunctiva, bulbar conjunctiva and conjunctiva of fornix were cultured by tissue inoculation in culture dish. The cell morphology and growth features were observed. Results The growth speeds of human conjunctival epithelium were different. The growth speeds of some places were faster than the other in one tissue, and the results of human conjunctival epithelium got from one location such as conjunctiva of fornix were similar. But the growth features of the tissue got from palpebral conjunctiva, bulbar conjunctiva and conjunctiva of fornix were similar. Conclusion Human conjunctival epithelium stem cells distribute in palpebral conjunctiva, bulbar conjunctiva and conjunctiva of fornix and the distribution is not uniform.