dbcAMP-Ca对内囊出血并应激性胃溃疡大鼠血浆和胃组织中PACAP的影响

Effect of dbcAMP-Ca on PACAP in plasma and stomach tissues of rats with gastric stress ulcers induced by internal capsule hemorrhage

目的探讨二丁酰环腺苷酸(dbcAMP-Ca)对大鼠内囊出血合并应激性胃溃疡的治疗作用及机制。方法用放射免疫分析(RIA)方法测定内囊出血大鼠dbcAMP-Ca治疗前后血浆及胃组织匀浆中垂体腺苷酸环化酶激活肽(PACAP)含量的变化。结果内囊出血时血浆PACAP明显高于对照组(P<0.01),胃组织匀浆中PACAP明显低于对照组(P<0.01);而用dbcAMP-Ca治疗后血浆PACAP明显低于内囊出血组(出血组)(P<0.01),胃组织匀浆中PACAP高于出血组(P<0.01)。结论血浆及胃组织匀浆中PACAP可能是参与内囊出血后应激性胃溃疡发生过程中的一个重要因素,dbcAMP-Ca对大鼠内囊出血并应激性胃溃疡有治疗作用。

Objective To investigate the therapeutic effects and mechanism of dbcAMP-Ca in treatment of rats with gastric stress ulcers induced by internal capsule hemorrhage. Methods The levels of pituitary adenylate cyclase activiting poly-peptide （PACAP） in plasma and stomach tissues of rats with cerebral hemorrhage and treated by dbcAMP-Ca after 24h were determined by RIA. Results The level of PACAP in plasma of cerebral hemorrhage group was significantly higher than that in control group （P〈0.01）, and the level of PACAP in stomach tissues of cerebral hemorrhage group was lower than that in control group （P〈0.01）. The level of PACAP in plasma of treatment group was significantly lower than that in cerebral hemorrhage group （ P 〈 0.01 ）. and the level of PACAP in stomach tissues of treatment group was higher than that in cerebral hemorrhage group （ P 〈 0. 01 ）. Conclusions PACAP may play some important role in gastric stress ulcers induced by internal capsule hemorrhage, and dbcAMP-Ca has an important effect in treatment of rats with gastric stress ulcers induced by internal capsule hemorrhage.