登革病毒速成甲基纤维素半微量空斑试验的建立

Quick semimicro plaque assay for dengue Ⅱ virus detection

目的：建立一种不需预先制备细胞单层的速成甲基纤维素半微量空斑技术。方法：以登革Ⅱ型病毒为代表，对速成法和常规法的实验条件、可靠性、敏感性、重复性等作系统研究。用方差分析和ｔ检验对实验结果进行统计学处理。结果：速成法重复性好，简单快速，敏感稳定，速成法空斑平均水平高于常规法（α１＞α２）。结论：速成甲基纤维素半微量空斑技术是一种可靠易行的病毒空斑新技术，可用于鉴定和滴定病毒，具有较大的实用和推广价值。

Objective:To establish a quick semimicro monolayer plaque assay for virus dectection (SQMPAV) without preparing monolayer in advance.Methods:Dengue Ⅱ virus was used to test with both SQMPAV and routine method and to study the experimental conditions,sensitivity,reproducibility of this SQMPAV method.The results were analysed with t test.Results:The titers of this SQMPAV method were higher than that of routine method(α 1>α 2).Conclusions:SQMPAV is a simple,rapid,reliable and feasible method and is of practical value in virological research.