摘要
目的:建立一种不需预先制备细胞单层的速成甲基纤维素半微量空斑技术。方法:以登革Ⅱ型病毒为代表,对速成法和常规法的实验条件、可靠性、敏感性、重复性等作系统研究。用方差分析和t检验对实验结果进行统计学处理。结果:速成法重复性好,简单快速,敏感稳定,速成法空斑平均水平高于常规法(α1>α2)。结论:速成甲基纤维素半微量空斑技术是一种可靠易行的病毒空斑新技术,可用于鉴定和滴定病毒,具有较大的实用和推广价值。
Objective:To establish a quick semimicro monolayer plaque assay for virus dectection (SQMPAV) without preparing monolayer in advance.Methods:Dengue Ⅱ virus was used to test with both SQMPAV and routine method and to study the experimental conditions,sensitivity,reproducibility of this SQMPAV method.The results were analysed with t test.Results:The titers of this SQMPAV method were higher than that of routine method(α 1>α 2).Conclusions:SQMPAV is a simple,rapid,reliable and feasible method and is of practical value in virological research.
出处
《蚌埠医学院学报》
CAS
1997年第1期55-56,共2页
Journal of Bengbu Medical College
关键词
登革病毒
空斑试验
甲基纤维素
dengue virus
plaque formation
methylcellulose