链脲佐菌素致糖尿病性勃起功能障碍大鼠模型的建立

Establishment of Diabetic Rats Models with Erectile Dysfunction Caused by Streptozotocin

目的:对链脲佐菌素(STZ)致大鼠糖尿病(DM)性勃起功能障碍(ED)模型建立进行研究。方法:30只SD大鼠,随机分为5组:对照组、注射STZ 40 mg/kg组、注射STZ 60 mg/kg组、注射STZ 80 mg/kg组、注射缓冲液组,每组6只,分别记录4 d、1周、2周、3周的空腹血糖、阿朴吗啡(APO)诱导阴茎勃起次数及体质量。结果:空腹血糖及体质量在不同四个时间点和不同处理组间差异均有显著性(P均<0.001),APO诱导阴茎勃起实验的勃起次数在注射STZ40 mg/kg组与注射STZ 60 mg/kg组间差异存在显著性(P=0.043)。结论:STZ致大鼠DM模型的最佳注射剂量应为60 mg/kg,APO诱导剂量以100μg/kg作为筛选ED模型的标准。

Objective：To study on diabetic rats models with erectile dysfunction caused by streptozotocin（STZ）. Methods： 30 male rates were randomly divided into five groups（control group .STZ 40mg/kg group. STZ 60 mg/kg group,STZ 80 mg/kg group,citrate buffer solution injection group）, 6 rats each group. And all rats were observed four days, one week, two weeks and three weeks after ,injected STZ. Fasting blood glucose, erectile frequency induced by apomorphine and change of mass should be recorded. Results Significant difference existed in fasting food glucose among groups of different test tim（ （P〈0. 001） and of different dose of STZ injection （P〈0. 001）. There was significant difference in erectile frequency induced by apomorphine between STZ 40mg/kg group and STZ 60 mg/kg group （P= 0. 043）. Conclusion ：The best dose of STZ injection to construct diabetes mellitus rats model is 60 mg/kg. Erectile dysfunction can not be considered if penile erection exists after APO injection, and the best dose of APO is 100μg/kg.