摘要
目的:建立补髓生血颗粒的含量测定方法。方法:采用RP-HPLC法测定补髓生血颗粒中淫羊藿苷(C33H40O15)的含量。使用C18柱,乙腈-甲醇-0.2mol/L磷酸水溶液(24∶9∶67)为流动相,检测波长为270nm。结果:淫羊藿苷的平均回收率为98.04%,RSD为1.9%。结论:方法稳定性、重复性好,结果准确、可靠。
Objective ： To establish method for determining the Content of Busui Shengxue Granules. Methods ： The content of Lcariin was determined by RP-HPLC. Lcariin was separated on spherisorb C18 column by acetonitrile-methol-0.2mol/L phosphoric acetic （ 24 ： 9 ： 67 ） as the mobile phase, the detection wavelength was 270nm. Results ： The average recovery was 98.04% and RSD was 1.9%.Conclusions ： The method was highly reproducible and accurate.
出处
《辽宁中医药大学学报》
CAS
2007年第3期191-192,共2页
Journal of Liaoning University of Traditional Chinese Medicine
