不同活化表型的巨噬细胞对淋巴管内皮细胞增殖、迁移和管样结构形成的影响

Effect of Macrophages with Different Activated Phenotypes on the Proliferation,Migration and Tube-like Structure Formation of Lymphatic Endothelial Cells

目的:探讨不同活化表型的巨噬细胞对淋巴管内皮细胞(LEC)增殖、迁移和管样结构形成的影响。方法:分别以mrIFN-γ、mrIL-4处理小鼠RAW264.7细胞,建立经典活化的巨噬细胞(caMphi)和替代性活化的巨噬细胞(aaMphi)模型。采用transwell系统,将活化的巨噬细胞和LEC共培养。通过绘制细胞生长曲线、细胞迁移实验和基质胶实验,分别观察它们对LEC增殖、迁移和管样结构形成的影响。结果:与aaMphi共培养的LEC增殖速度较快,迁移能力和管样结构形成能力均明显强于其它对照组。结论:aaMphi能够促进LEC增殖、迁移和管样结构形成。

Objective： To explore the effect of macrophages with different activated phenotypes on the proliferation, migration and tube-like structure formation of lymphatic endothelial cells （LEC）.Methods： Classically activated macrophages （caMphi） and alternatively activated macrophages （aaMphi） models were established by RAW264.7 macrophages treated with mrIFN-γ and mrIL-4. Based on the co-culture systems of activated macrophages and LEC, cell growth curve, migration assay and matrigel assay were used to observe the effect of macrophages on the proliferation,migration and tube-like structure formation of LEC. Results. The proliferation of LEC co-cultured with aaMphi was faster, and their ability to migrate and form tube-like structure was signif-icantly stronger than that in the other groups. Conclusion. aaMphi can promote the proliferation,migration and tube-like structure formation of LEC.