生物胶用于修复家兔尿道下裂的作用机制

Mechanism of Alpha-cyanoacrylateglue in the Repairing of Hypospadias in Rabbits

目的:研究α-氰基丙烯酸酯胶黏合修补兔尿道下裂的作用机制。方法:20只雄性尿道下裂模型兔采用用Snodgrass术修复,尿道采用生物胶黏合,皮肤采用缝合的方法,分别于术后1,2,6周处死,检查尿道并作连续石蜡切片,应用HE染色进行组织病理学评价,术后6周动物在处死前行逆行尿道造影检查有无尿道狭窄发生。结果:在整个实验过程中无动物死亡,术中尿道逆行注美蓝无外漏,术后2周发生尿瘘1例,发生率为5%;逆行尿道造影检查结果显示生物胶黏合后尿道形状恢复完好,无尿道狭窄发生。阴茎连续病理组织切片示尿道黏膜由于受生物胶的阻碍较皮肤愈合慢,显示与常规缝合不同的修复机制。结论:应用生物胶进行尿道下裂的修复,由于水封性好,愈合机制的改变,有利于降低尿瘘的发生率。

Objective： To study the mechanism of α-cyanoacrylateglue in repairing rabbit hypospadias models. Methods. Male rabbits （n=20） underwent an incised plate urethroplasty. All the models were killed and urethras were examined at 1, 2 and 6 week（s） postoperatively. Serial sections of the penis were stained with haematoxylin and eosin for histological evaluation. A retrograde urethrogram was made at 6 weeks postoperatively inspected for urethral stricture. Results. There were no animal dead in the whole experiment. One fistula occurred at 2 weeks postoperatively and the incidence of fistula was 5 %. Retrograde urethrogram showed no stricture. Microscopic examination indicated a different mechanism of healing in α-cyanoacrylateglue reparing models, that the healing of skin was prior to that of urothelium. Conclusion. Preliminary results suggest that using watertight bioglue would have low incidence of urethra fistula and urethral stricture.