板蓝根多糖对宿主抵抗鼠伤寒沙门菌感染能力的影响

Research of Resistance to Salmonella Typhimurium Infection Using Banlangen Polysaccharide

目的:观察板蓝根多糖注射鼠伤寒沙门菌感染的小鼠后,对小鼠抵抗鼠伤寒杆菌能力的影响。方法:水煮法提取板蓝根多糖,配成悬液,腹腔注射感染鼠伤寒的小鼠,观察小鼠的生存时间,脾脏的重量指数,脏器菌落计数,检测小鼠血清中抗体IgG水平,用ELISA法测定小鼠脾脏释放的细胞因子。结果:注射板蓝根多糖的小鼠,生存时间延长,脾脏的重量指数,菌落计数均比对照组明显降低,血清中抗体IgG水平升高,脾脏细胞分泌细胞因子IFN-γ水平上升。结论:板蓝根多糖能够通过增强抗体的产生,调节细胞因子的释放,增强宿主的体液免疫和细胞免疫功能,保护宿主抵抗胞内菌的感染。

Objective： To demonstrate whether banlangen polysaccharide could inhibit the infection of Salmonella typhimurium in mice. Methods.. Banlangen polysaccharide was extracted with boiling methods. BALB/c mice treated with banlangen polysaccharide were injected via intraperitoneally for three days successively, while the control mice were treated with PBS. After three days, every mouse was infected with Salmonella typhimurium C5. The survival rate of mice was recorded. The numbers of viable bacteria in the spleen was counted and the WI （weight index）of spleen,level of IgG in mouse sera and level of IFN-γ in mouse spleens were measured. Results： In banlangen polysaccharide group, survival rate of mice was higher than in control group. The numbers of viable bacteria in spleen and WI of spleen were much lower than control group. The levels of IgG and IFN-γ were also increased in banlangen polysaccharide group. Conclusion： Banlangen polysaccharide can protect host against Salmonella typhimurium C5 infection by enhancing humoral immunity and cellular immunity.