折光法快速判断中药柱层析过程中的始终点及其在树脂精制丹参有效部位中的应用

Refractive principle to judge the beginning point and the end point of the process of the traditional Chinese medicine filtered by colume Chromatography rapidly and their application in purification active region of Dan Shen Root with macroporous resin

目的:介绍运用折光原理快速判断中药柱层析过程中始终点的方法及其在大孔树脂精制丹参有效部位的应用。方法:通过丹参提取物经AB-8型大孔吸附树脂精制有效部位,水-30%乙醇-80%乙醇梯度洗脱,间隔取样,在线监测洗脱液折光率并分析变化规律,同时以高效液相色谱法和紫外可见分光光度法分别检测丹酚酸B、丹参萜类成分含量作参比,判断洗脱过程的始点和终点。结果:丹参有效部位精制过程中洗脱的始终点为:水洗终点第4BV(折光率0),即30%乙醇洗脱始点,30%乙醇洗脱终点第8BV(折光率10.5),即80%乙醇洗脱始点,80%乙醇洗脱终点第10.75BV(折光率19.5)。结论:折光法准确可靠,便捷快速,在中药柱层析处理过程有很强的实践意义。

Object：introduce the method applying refractive principle to judge thebeginning point and the end point of the process of the traditional Chinese medicine filtered by colume Chromatography rapidly and their application in purification active region of Dan Shen Root with macroporous resin separation technique. Method： by refining the active region of Dan Shen Root with the AB- 8 type of macroporous resin, gradient elution of water- 30% alcohol- 80% alcohol, interval sampling, monitor the refractive index of eluant on- line, analyze its regularity and judge the beginning point and the end point of the elution process. At the same time, high performance liquid chromatography and ultraviolet- spectrophotometry are usded to measure the contents of salvianolic acid B and Terpene, which can be taken as a reference. Result： the beginning point and the end point of the eluotropic process for the active region of Dan Shen Root： terminal point for water is 4th BV （refractive index：0）, but the beginning point for 30% alcohol, terminal point for 30% alcohol is 8th BV （refractive index： 10.5）, but the beginning point for 80% alcohol, the terminal point for 80% alcohol is 11.5th BV（refractive index： 19.5）.Conclusion： this method is not only accurate and reliable , but also convenient and＇rapid, thus it has very strong practic significance in the process of the traditional Chinese medicine filtered by colurne Chromatography.