栀子及其近缘类群的随机扩增多态DNA分析

RAPD Analysis for Gardenia jasminoides and Relatives

目的:探索栀子与其变种雀舌栀子、重瓣栀子及变型水栀子之间的亲缘关系。方法:从56个随机引物中筛选出12个引物对栀子及其近缘类群进行随机扩增多态DNA分析,应用SPSS10.0分析软件中的Jaccard方法计算任意两样品间的相似系数,用组内聚类方法计算得出聚类树状图。结果:共得到251个有效位点。聚类分析显示,栀子与重瓣栀子最先聚类,然后与水栀子聚类,最后与雀舌栀子聚类。结论:重瓣栀子与栀子亲缘关系最近,雀舌栀子与栀子的亲缘关系最远。

Objective： To probe relative relationship among Gardenia jasminoides, its varietas（ G. jasminoides Ellis. var. radicans and G . jasminoides Ellis. vat. fortuniana ） and its forma （ G. jasminoides Ellis. f . longicarpa ）. Methods： RAPD analysis for samples of Gardenia jasminoides and relatives were performed by 12 random primers screened from 56 Proximity matrix was analyzed by SPSS version 10.0 software and rescaled distance cluster combine was obtained by cluster analysis within groups. Results： A total of 251 polymorphic loci were amplified. It showed that Gardenia jasminoides were clustered with G .jasminoides Ellis. var.fortuniana at first, then with G. jasminoides Ellis. f . longicarpa , and with G. jasminoides Ellis. var . radicans at last. Conclusions： Among samples, Gardenia jasminoides was the closest relative to G.jasminoides Ellis.var.fortuniana, and most distant relative to G. jasminoides Ellis. var . radicans .