均匀设计对藁本药材中阿魏酸含量测定方法的改进

The Amelioration of Ferulic Acid Analysis of Rhizoma Ligustici with Uniform Design

目的:改进藁本药材中有效成分阿魏酸的含量测定方法。方法:采用均匀设计安排试验,对藁本颗粒大小、提取溶剂的量、提取时间等条件进行考察,得出最佳的提取条件。结果:藁本中阿魏酸的含量测定以药材粉末过药典6号筛,水浴回流提取2小时的方法为最佳。结论:改进后得到的最佳提取方式可以更真实地反映出藁本药材中阿魏酸的含量。

Objective： By taking reversed phased high performance liquid chromatography and uniform design, study and ameliorate the ferulic acid assay of Rhizoma Ligustici. Methods： By comparing the extracting method, granule size, extracting solvent and extracting time, we got the optimum extracting condition. Results： the optimum extracting condition was water bath re - circulating extract for 2 hours, the granule size pass through 100 mush. Conclusion： the ameliorated extracting method could improve the extracting efficiency greatly, and evaluate the quality of raw Rhizoma Ligustici more truly.