韩国栗疫病抗病品种的遗传变异分析

Genetic variation of chestnut cultivars for resistance to Cryphonectria parasitica in Korea

为了分析韩国栗疫病的抗病品种和感病品种的遗传变异和抗病性的筛选,利用抗病性的快速检测法和RAPD(random amplified polymorphic DNA)方法对13个栗树品种进行了抗病性检测和RAPD标记分析。抗病性的快速检测选出了5个抗病品种、5个感病品种和3个中度抗病(或中度感病)品种,并且这一结果与该品种的田间表现相一致。利用筛选的12个随机引物,扩增了100个多态性RAPD片段,但未发现与抗病性或感病性相关的特异RAPD片段。聚类分析结果表明,12个品种大致分为抗病、感病和中度抗病(或中度感病)等3个大组,并与抗病性的快速检测结果基本一致。抗病品种“MANSEKI”表现出了相对于12个品种较远的亲缘关系。

Resistance of 13 chestnut cultivars （ Castanea spp. ） to Cryphonectria parasitica in Korea was determined by the rapid testing method. Five chestnut cultivars are resistant, five cultivars susceptible and three cultivars moderate resistant （or susceptible）. The genetic variation was investigated by the random amplified polymorphie DVA（RAPD）, 12 random primers Were selected in RAPD analysis, 100 polymorphic RAPD bands were amplified, but the peculiar RAPD band which discriminates between resistant and susceptible cultivars could not be detected. For the linkage result of cluster analysis, 12 vars could be classified as the resistant, susceptible and moderate resistant （or susceptible） groups approximately, which are almost coordinated to the above rapid testing results. The genetic relationship between one resistant cultivar and other 12 cultivars is very tow. These results suggest that the primers selection of genetic regions related to resistance will be effective for the research of genetic variation and identification of resistance in chestnut.