切叶蚁亚科七属十二种的分子系统学研究

Molecular systematic study on twelve species of seven genera in Myrmicinae (Hymenoptera:Formicidae) from Guangxi,South China

测定了切叶蚁亚科7属12种的线粒体CO1、CO2的部分基因及完整的tRNALeu基因DNA序列,对DNA序列进行了分析,对tRNALeu基因进行了二级结构分析;根据DNA序列数据和氨基酸序列数据,以臭蚁亚科的Forelius chalybaeus作为外群,采用最大似然法(ML)、最大简约法(MP)、邻接法(NJ)、未加权组对算术平均法(UPGMA)构建分子系统树,通过自举检验,得到自举置信水平,以此检验该分子系统树的可靠性。研究结果显示,基于以上基因的分子系统分析与传统分类分析的结果基本一致,且在属级的一致性高于种级的一致性。

Cytochrome oxidase subunits 1 （CO1） and 2 （CO2）, and the entire sequence of the gene for tRNA leucine from twelve species of seven genera of the ant subfamily Myrmicinae were sequenced. The DNA sequences were analyzed, and the secondary structure of tRNA leucine also gene was studied. The molecular phylogenetic analyses of the subfamily Myrmicinae with Forelius chalybaeus, a species of the subfamily Dolichoderinae as the outgroup were performed by using four methods： the maximum likelihood （ML）, maximum parsimony （MP）, neighbor-joined （N J）, and unweighted pairgroup method with arithmetic means （UPGMA） based on the deoxyribonucleic acid data and the amino acid data. The taxonomic scheme for these genera and species based on molecular phylogeny of above genes consisted with that of the traditional taxonomy and the consistency for genera was better than that for species.