中国不同地域恒河猴MHC-Ⅰ型部分等位基因的调查

Typing of Several MHC-Ⅰ Alleles of Rhesus Monkeys Derived from Different Regions of China

采用序列特异性引物-聚合酶链式反应(PCR-SSP)分型方法对在华南灵长类动物研究中心繁殖的247只中国恒河猴(Macaca mulatta)(其中30只来源于广西、34只来源于海南,183只来源于川西、安徽等内陆地区杂交群)的Mamu-A01、A02、A08、B01和NA7等5个MHC-Ⅰ型分子位点进行检测。结果显示,A01、A02发现于海南群和杂交群,阳性率均小于8.8%;A08发现于杂交群中,阳性率小于3.8%;B01发现于广西群和杂交群,阳性率均大于17.48%;NA7在3个猴群中均有发现,阳性率均大于32.4%。中国不同地域恒河猴群体携带的5个MHC-Ⅰ型等位基因的频率存在明显差异,通过与印度的恒河猴比较,中国恒河猴与印度恒河猴携带的MHC-Ⅰ等位基因也存在显著的差异。

PCR-SSP was applied to type five MHC- Ⅰ alleles （Mamu-A ＊ 01, A ＊ 02, A ＊ 08, B ＊ O1 and NA7 ） of Chinese Rhesus Monkeys （Macaca mulatta ）derived from Hainan （n = 30）, Guangxi （n = 34） and Crossbreed groups （ n = 183） .The results showed that A ＊ 01 and A ＊ 02 existed in Hainan and Crossbreed groups with frequencies less than 8.8% , A ＊ 08 only existed in crossbreed group with a frequency less than 3.8% . The frequencies of B ＊ 01 in Guangxi and Crossbreed groups were more than 17.48% , while NA7 was found in Guangxi, Hainan and Crossbreed groups with frequencies more than 32.4%. The different allelic frequencies among monkeys derived from different regions of China suggest that there are great differences in distribution frequencies of MHC- Ⅰ alleles among them. In addition, compared to Indian Rhesus Monkey, this discrepancy also appeared in Chinese Rhesus Monkey and Indian Rhesus Monkey.