摘要
报道了虎纹捕鸟蛛(Ornithoctonus huwena)食道下神经节的解剖以及神经细胞的分离培养方法。为开展蜘蛛神经生物学实验方法的研究,创立了一种蜘蛛神经细胞解剖方法——去胸甲解剖法。神经节细胞培养基为:NaCl 223 mmol/L;KCl 6.8mmol/L;CaCl2 8mmol/L;MgCl2 5.1mmol/L;Sucrose 5mmol/L;Hepes 10mmol,L;谷氨酰胺1mmol/L;青霉素200IU/ml;链霉素200μg/ml;小牛血清20%;pH7.4。在温度(27±2)℃的培养箱中培养2~4h。实验结果表明:与传统方法相比较,去胸甲解剖法具有取材简便、准确、快捷和高效的特点。改进的培养基非常适合蜘蛛离体神经细胞的培养。培养的细胞状态好、数量多,细胞体呈椭圆形,细胞形状近似汤勺,有一个长的单极突起,其大小为10~30μm。
The anatomy, dissociation and culture of neurons isolated from the subesophageal ganglion (SUB) of the spider Ornithoctonus huwena, are described. A new method, the anatomical method of removing the Sternum, to anatomize the spider ganglion was created. The culture media used for this study contained: NaCl 223 mmol/L; KC1 6.8 mmol/L; CaCl 2 8 mmol/L; MgCl2 5.1 mmol/L; Sucrose 5 mmol/L; Hepes 10 mmol/L; Glutamine 1 mmol/L; Penicillin 200 IU/ml; Streptomycin 200 μg/ml; Bovin Calf Serum 20% ; pH 7.4. The culture temperature was at 27 ± 2℃ and it took about 2 - 4 h for the process. The experimental results demonstrate that: the culture media is fit for the dissociated spider nerve cells. Most cells are in good condition, and above 70% cells survive in the cell culture dishes. The soma of the nerve cell shaped like an ellipse and the neural cell body which has a single axon shaped like a spoon. The size of those cells varies from 10 to 30μm.
出处
《动物学杂志》
CAS
CSCD
北大核心
2007年第2期63-67,共5页
Chinese Journal of Zoology
基金
国家自然科学基金资助项目(No.39570119
30370208)
关键词
虎纹捕鸟蛛
食道下神经节
去胸甲解剖法
细胞培养
Ornithoctonus huwena
The subesophageal ganglion
The anatomical method of removing the sternum
Cell culture