蜘蛛神经节的解剖与神经细胞的分离培养

Anatomy of Spider Ganglion and Culture of the Spider Neurons

报道了虎纹捕鸟蛛（Ornithoctonus huwena）食道下神经节的解剖以及神经细胞的分离培养方法。为开展蜘蛛神经生物学实验方法的研究，创立了一种蜘蛛神经细胞解剖方法——去胸甲解剖法。神经节细胞培养基为：NaCl 223 mmol／L；KCl 6．8mmol／L；CaCl2 8mmol／L；MgCl2 5．1mmol／L；Sucrose 5mmol／L；Hepes 10mmol，L；谷氨酰胺1mmol／L；青霉素200IU/ml；链霉素200μg／ml；小牛血清20％；pH7．4。在温度（27±2）℃的培养箱中培养2～4h。实验结果表明：与传统方法相比较，去胸甲解剖法具有取材简便、准确、快捷和高效的特点。改进的培养基非常适合蜘蛛离体神经细胞的培养。培养的细胞状态好、数量多，细胞体呈椭圆形，细胞形状近似汤勺，有一个长的单极突起，其大小为10～30μm。

The anatomy, dissociation and culture of neurons isolated from the subesophageal ganglion （SUB） of the spider Ornithoctonus huwena, are described. A new method, the anatomical method of removing the Sternum, to anatomize the spider ganglion was created. The culture media used for this study contained： NaCl 223 mmol/L; KC1 6.8 mmol/L; CaCl 2 8 mmol/L; MgCl2 5.1 mmol/L; Sucrose 5 mmol/L; Hepes 10 mmol/L; Glutamine 1 mmol/L; Penicillin 200 IU/ml; Streptomycin 200 μg/ml; Bovin Calf Serum 20% ; pH 7.4. The culture temperature was at 27 ± 2℃ and it took about 2 - 4 h for the process. The experimental results demonstrate that： the culture media is fit for the dissociated spider nerve cells. Most cells are in good condition, and above 70% cells survive in the cell culture dishes. The soma of the nerve cell shaped like an ellipse and the neural cell body which has a single axon shaped like a spoon. The size of those cells varies from 10 to 30μm.