铜、镉及其复合作用下中华大蟾蜍蝌蚪Na^+-K^+-ATPase酶的变化

Response of the Na^+-K^+-ATPase Activity of Bufo gargarizans Tadpole to Cu^(2+)、Cd^(2+) Single and Combined Pollution Exposure

采用动物毒理实验法,研究了铜、镉及其复合污染对中华大蟾蜍(Bufo gargarizans)蝌蚪Na+-K+-ATPase酶活性的影响。结果表明,在0.0298mg/LCu2+和1.14mg/LCd2+作用下,蝌蚪Na+-K+-ATPase酶活性极显著高于对照组(P<0.01)。随着Cu2+、Cd2+暴露浓度的增加,蝌蚪Na+-K+-ATPase酶活性又逐渐降低,甚至被抑制。铜、镉复合污染在Na+-K+-ATPase酶水平上表现出一定的协同作用。

The effects of Cu^2＋, Cd^2＋ single and combined pollution on the Na^＋ -K^＋ -ATPase activities in Bufo gargarizans tadpoles were tested by the method of animal toxicological experiment. The results showed that the Na^＋ - K^＋ -ATPase activities of tadpoles increased significantly at the group of 0.029 8 mg/L Cu^2＋ and 1.14 mg/L Cd^2＋ comparing to the control group. But with the increasing of Cu^2＋ and Cd^2＋ concentration, the damage of tadpoles became much heavier; the Na^＋ -K^＋ -ATPase activity was gradually decreased and was even inhibited significantly. The joint effects of the combined pollution on the Na^＋ -K^＋ - ATPase activity were synergistic.