摘要
背景与目的:研究富硒益生菌的急性毒性和遗传毒性。材料与方法:采用小鼠急性毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验等对富硒益生菌进行安全性试验研究。结果:急性毒性试验表明,小鼠灌胃给予富硒益生菌,其LD50为18.49g/kg。Ames试验、微核试验和精子畸形试验结果均为阴性。结论:在本次实验条件下,富硒益生菌表现出一定的急性毒性,但未见遗传毒性。由此可初步判定,在人体推荐摄入剂量每天50mg范围内使用富硒益生菌是安全可靠的。
BACKGROUND ＆ AIM： To study the acute toxicity and genotoxicity of selenium-enriched probiotics. MATERIALS METHODS： Acute toxicity test of mice, Ames test, micronucleus test of bone marrow PCE cell in mice, sperm abnormality test of mice were used. RESULTS： The oral acute toxicity study revealed that the LD50 of selenium-enriched probiotics was 18.49 g/kg in mice. The results of genotoxicity tests were all negative, including Ames test, micronucleus test and sperm shape abnormality test. CONCLUSION： .Selenium-enriched probiotics showed certain toxicity but no genotoxicity in this study. Thus, selenium-enriched probiotics was safe at the daily-recommended dose（50 mg/person/day） and has the potential for development into functional foods.
出处
《癌变．畸变．突变》
CAS
CSCD
2007年第2期96-98,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金项目(30371060
30671547)
江苏省自然科学基金(BK2006144)
关键词
富硒益生菌
急性毒性
AMES试验
微核试验
精子畸形试验
selenium-enriched probiotics
acute toxicity
Ames test
micronucleus test
sperm abnormality test
